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  • Featured Product
  • KD/KO Validated

APPBP1 Polyclonal antibody

APPBP1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14863-1-AP

Print datasheet

Synonyms

A 116A10.1, APP BP1, APPBP1, HPP1, NAE1, Proto oncogene protein 1, ula 1

验证数据展示

  • WB analysis of human kidney using 14863-1-AP

    WB analysis of human kidney using 14863-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 14863-1-AP

    IP experiment of mouse kidney using 14863-1-AP

    IP result of anti-APPBP1 (IP:14863-1-AP, 4ug; Detection:14863-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman kidney tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14863-1-AP targets APPBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen APPBP1 fusion protein Ag6672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NEDD8 activating enzyme E1 subunit 1
Synonyms A 116A10.1, APP BP1, APPBP1, HPP1, NAE1, Proto oncogene protein 1, ula 1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000480
Gene Symbol NAE1
Gene ID (NCBI) 8883
RRIDAB_2251388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APPBP1 antibody 14863-1-APDownload protocol
IP protocol for APPBP1 antibody 14863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Polo-like kinases mediate cell survival in mitochondrial dysfunction.

Authors - Takumi Matsumoto
  • KD Validated
View Article