Neurogranin Polyclonal antibody

Neurogranin Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10440-1-AP

Print datasheet

Synonyms

hng, Neurogranin, Ng, NRGN, P17, RC3



经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10440-1-AP targets Neurogranin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Neurogranin fusion protein Ag0676 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurogranin (protein kinase C substrate, RC3)
Synonyms hng, Neurogranin, Ng, NRGN, P17, RC3
Calculated Molecular Weight 7.6 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC002835
Gene Symbol NRGN
Gene ID (NCBI) 4900
RRIDAB_2251586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92686
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.The observed molecular weight of human NRGN by Western blotting is about 15-17 kDa.

实验方案

Product Specific Protocols
WB protocol for Neurogranin antibody 10440-1-APDownload protocol
IHC protocol for Neurogranin antibody 10440-1-APDownload protocol
IP protocol for Neurogranin antibody 10440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cells

Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.

Authors - Marina Di Domenico
mouseIHC

Sci Rep

Hypoxia-inducible factor-2α is crucial for proper brain development.

Authors - Kira Kleszka
mouse

Elife

An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning.

Authors - Ingrid Ehrmann