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  • KD/KO Validated

OAS1/3 Polyclonal antibody

OAS1/3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14955-1-AP

Print datasheet

Synonyms

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OAS1/3, OIAS, OIASI, p46/p42 OAS



经过测试的应用

Positive WB detected inIFN alpha treated HeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14955-1-AP targets OAS1/3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Immunogen OAS1/3 fusion protein Ag6793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2',5'-oligoadenylate synthetase 1, 40/46kDa
Synonyms (2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OAS1/3, OIAS, OIASI, p46/p42 OAS
Calculated Molecular Weight 46 kDa
Observed Molecular Weight ~40 kDa, 100-120 kDa
GenBank Accession NumberBC000562
Gene Symbol OAS1
Gene ID (NCBI) 4938
RRIDAB_2158292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have cross-reaction with OAS3 protein due to the high homology.

实验方案

Product Specific Protocols
WB protocol for OAS1/3 antibody 14955-1-APDownload protocol
IHC protocol for OAS1/3 antibody 14955-1-APDownload protocol
IF protocol for OAS1/3 antibody 14955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

A prenylated dsRNA sensor protects against severe COVID-19.

Authors - Arthur Wickenhagen
  • KO Validated
human

Adv Sci (Weinh)

The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.

Authors - Qixue Wang
humanWB

PLoS Biol

Resurrection of 2'-5'-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication

Authors - Spyros Lytras
mouseWB

Autophagy

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Authors - Xibao Zhao
humanWB

J Invest Dermatol

OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.

Authors - Yan-Zhou Huang
  • KD Validated
humanWB,IHC

Elife

A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.

Authors - Jia Li