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CoraLite®594-conjugated PADI2 Monoclonal antibody

PADI2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8C12

Cat No : CL594-66386

Print datasheet

Synonyms

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

验证数据展示

  • IF Staining of human breast cancer using CL594-66386

    IF Staining of human breast cancer using CL594-66386

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66386 (PADI2 antibody) at dilution of 1:50.

  • FC experiment of HeLa using CL594-66386

    FC experiment of HeLa using CL594-66386

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PADI2 (CL594-66386, Clone:2D8C12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-66386

    IF Staining of HeLa using CL594-66386

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PADI2 antibody (CL594-66386, Clone: 2D8C12 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman breast cancer tissue, HeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66386-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66386 targets PADI2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen PADI2 fusion protein Ag17612 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name peptidyl arginine deiminase, type II
Synonyms KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II
Calculated Molecular Weight 665 aa, 75 kDa
GenBank Accession NumberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
RRIDAB_2883573
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2J8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa. This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 PADI2 antibody CL594-66386Download protocol
FC protocol for CL594 PADI2 antibody CL594-66386Download protocol
Standard Protocols
Click here to view our Standard Protocols