PAWR Polyclonal antibody
PAWR Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PAWR Ready-To-Use IHC Kit. PAWR primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
20688-1-AP targets PAWR in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, WB,ELISA |
| Cited Applications | IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PRKC, apoptosis, WT1, regulator |
| Synonyms | Par 4, PAR4, PAWR, Prostate apoptosis response 4 protein |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | NM_002583 |
| Gene Symbol | PAWR |
| Gene ID (NCBI) | 5074 |
| RRID | AB_10733473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96IZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAWR, also named as PAR4, is a pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. It induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. PAWR inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. It down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. PAWR may be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. The antibody is specific to PAWR.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAWR antibody 20688-1-AP | Download protocol |
| IHC protocol for PAWR antibody 20688-1-AP | Download protocol |
| FC protocol for PAWR antibody 20688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. | ||
