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ERp57/ERp60 Polyclonal antibody
ERp57/ERp60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, L02 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15967-1-AP targets ERp57/ERp60 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | ELISA, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | ERp57/ERp60 fusion protein Ag8741 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein disulfide isomerase family A, member 3 |
Synonyms | 58 kDa microsomal protein, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp57/ERp60, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3 |
Calculated Molecular Weight | 505 aa, 57 kDa |
Observed Molecular Weight | 57 kDa |
GenBank Accession Number | BC014433 |
Gene Symbol | PDIA3 |
Gene ID (NCBI) | 2923 |
RRID | AB_2236784 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P30101 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
IHC protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
IF protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
IP protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Biol Evol Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. | ||
Redox Biol Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress. | ||
EMBO J TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. | ||
Cell Rep Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes. | ||
J Clin Endocrinol Metab Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure. |