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PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 23274-1-AP

Print datasheet

Synonyms

BRPK, PARK6, PINK1, PTEN induced putative kinase 1



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23274-1-AP targets PINK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, rabbit, zebrafish, pig, duck
Immunogen PINK1 fusion protein Ag19825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PTEN induced putative kinase 1
Synonyms BRPK, PARK6, PINK1, PTEN induced putative kinase 1
Calculated Molecular Weight 581 aa, 63 kDa
Observed Molecular Weight 65 kDa, 45 kDa
GenBank Accession NumberBC028215
Gene Symbol PINK1
Gene ID (NCBI) 65018
RRIDAB_2879244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9BXM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

实验方案

Product Specific Protocols
WB protocol for PINK1 antibody 23274-1-APDownload protocol
IHC protocol for PINK1 antibody 23274-1-APDownload protocol
IF protocol for PINK1 antibody 23274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu
humanWB

Nucleic Acids Res

DNA damage invokes mitophagy through a pathway involving Spata18.

Authors - Xiuli Dan
mouseWB

Autophagy

Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development.

Authors - Rei Unno
mouseWB, IHC

Autophagy

Autophagy regulates functional differentiation of mammary epithelial cells.

Authors - Jessica Elswood
humanIHC,IF

Autophagy

CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma.

Authors - Jingyue Yao
mouseWB

Autophagy

Restoration of mitophagy ameliorates cardiomyopathy in Barth syndrome.

Authors - Jun Zhang