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POLR3E Polyclonal antibody

POLR3E Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15022-1-AP

Print datasheet

Synonyms

KIAA1452, POLR3E, RNA polymerase III subunit C5, RPC5, SIN

验证数据展示

  • WB analysis of PC-3 using 15022-1-AP

    WB analysis of PC-3 using 15022-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15022-1-AP (POLR3E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 15022-1-AP

    IP experiment of PC-3 using 15022-1-AP

    IP result of anti-POLR3E (IP:15022-1-AP, 5ug; Detection:15022-1-AP 1:300) with PC-3 cells lysate 3200ug.

经过测试的应用

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15022-1-AP targets POLR3E in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen POLR3E fusion protein Ag7098 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide E (80kD)
Synonyms KIAA1452, POLR3E, RNA polymerase III subunit C5, RPC5, SIN
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC000285
Gene Symbol POLR3E
Gene ID (NCBI) 55718
RRIDAB_2878101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVU0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLR3E antibody 15022-1-APDownload protocol
IP protocol for POLR3E antibody 15022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols