PR repeat Polyclonal antibody

PR repeat Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

ELISA and More (4)

Conjugate

Unconjugated

Cat No : 23979-1-AP

Print datasheet

Synonyms

C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP



经过测试的应用

Positive ELISA detected inpeptide

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23979-1-AP targets PR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Applications ELISA
Cited Applications Dot Blot, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, Drosophila
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR repeat
Synonyms C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP
Gene Symbol
Gene ID (NCBI)
RRIDAB_2879388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.

发表文章

SpeciesApplicationTitle
humanIHC

Nat Neurosci

RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.

Authors - Shizuka B Yamada
ratWB, IF

Neuron

Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.

Authors - Xinmei Wen
mouseIF

Nat Commun

Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR.

Authors - Zongbing Hao
humanWB, IHC

Acta Neuropathol

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Authors - Ian R A Mackenzie
humanWB

EMBO J

NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification.

Authors - Qing Tang
humanWB

EMBO J

The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.

Authors - Yuya Kawabe