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PRDM10 Polyclonal antibody

PRDM10 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 23827-1-AP

Print datasheet

Synonyms

KIAA1231, PFM7, PR domain containing 10, PRDM10, TRIS, Tristanin

验证数据展示

  • WB analysis of HEK-293 using 23827-1-AP

    WB analysis of HEK-293 using 23827-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23827-1-AP (PRDM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 23827-1-AP

    IP experiment of mouse testis using 23827-1-AP

    IP result of anti-PRDM10 (IP:23827-1-AP, 5ug; Detection:23827-1-AP 1:500) with mouse testis tissue lysate 8000ug.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23827-1-AP is PRDM10 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen PRDM10 fusion protein Ag20605 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR domain containing 10
Synonyms KIAA1231, PFM7, PR domain containing 10, PRDM10, TRIS, Tristanin
Calculated Molecular Weight 1156 aa, 131 kDa
Observed Molecular Weight 120-150 kDa
GenBank Accession NumberBC112934
Gene Symbol PRDM10
Gene ID (NCBI) 56980
RRIDAB_2879330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDM10 is a member of PRDM family, which contains a PR(PRDI-BF1 and RIZ homology) domain, and PRDM is also a subgroup of PR/SET transcription factor family for its PR domain is similar to the catalytic motif of the SET domain of histone methyltransferase(HMTase). Thus PRDM is suggested involve in the regulation of genome expression and chromatin remodeling. PRDM10 shares characteristics with the PR/SET faimly and clusters of zinc fingers DNA binding motifs. It's postualated PRDM10 has an essential role in regulating gene expression and tissue differentiation and a gene repressor. It may play a role in the pathogenesis of gangliosidosis.

实验方案

Product Specific Protocols
WB protocol for PRDM10 antibody 23827-1-APDownload protocol
IP protocol for PRDM10 antibody 23827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols