CD133-1,2,4,7 Polyclonal antibody

CD133-1,2,4,7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 19945-1-AP

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Synonyms

AC133, Antigen AC133, CD133, CD133-1,2,4,7, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4



经过测试的应用

Positive WB detected inY79 cells, mouse lung tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19945-1-AP targets CD133-1,2,4,7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prominin 1
Synonyms AC133, Antigen AC133, CD133, CD133-1,2,4,7, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberNM_001145851
Gene Symbol PROM1
Gene ID (NCBI) 8842
RRIDAB_10644154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2,4,7 (UNIPROT has updated the info of isoforms)of CD133.

实验方案

Product Specific Protocols
WB protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IHC protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IP protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols