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PTBP1 Polyclonal antibody

PTBP1 Polyclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55181-1-AP

Print datasheet

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

验证数据展示

  • IHC staining of mouse brain using 55181-1-AP

    IHC staining of mouse brain using 55181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55181-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • WB analysis of HepG2 using 55181-1-AP

    WB analysis of HepG2 using 55181-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55181-1-AP (PTBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 55181-1-AP

    WB analysis of HeLa using 55181-1-AP

    WB result of PTBP1 antibody (55181-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

  • IF Staining of mouse brain using 55181-1-AP

    IF Staining of mouse brain using 55181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55181-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55181-1-AP targets PTBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polypyrimidine tract binding protein 1
Synonyms HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 55-62 kDa
GenBank Accession NumberNM_002819
Gene Symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_11182384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

实验方案

Product Specific Protocols
WB protocol for PTBP1 antibody 55181-1-APDownload protocol
IHC protocol for PTBP1 antibody 55181-1-APDownload protocol
IF protocol for PTBP1 antibody 55181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
View Article