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  • KD/KO Validated

RAB10 Polyclonal antibody

RAB10 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27094-1-AP

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Synonyms

RAB10, Ras related protein Rab 10



经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27094-1-AP targets RAB10 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen RAB10 fusion protein Ag25830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB10, member RAS oncogene family
Synonyms RAB10, Ras related protein Rab 10
Calculated Molecular Weight 200 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000896
Gene Symbol RAB10
Gene ID (NCBI) 10890
RRIDAB_2880752
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61026
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB10 antibody 27094-1-APDownload protocol
IHC protocol for RAB10 antibody 27094-1-APDownload protocol
IF protocol for RAB10 antibody 27094-1-APDownload protocol
FC protocol for RAB10 antibody 27094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
mouseIF

Biochem Biophys Res Commun

Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation

Authors - Miku Fukawa