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  • Featured Product
  • KD/KO Validated

RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 27403-1-AP

Print datasheet

Synonyms

RAB5B, Ras related protein Rab 5B

验证数据展示

  • WB analysis of HeLa using 27403-1-AP

    WB analysis of HeLa using 27403-1-AP

    WB result of RAB5B antibody (27403-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB5B transfected HeLa cells.

  • WB analysis of mouse lung using 27403-1-AP

    WB analysis of mouse lung using 27403-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 27403-1-AP

    IF Staining of NIH/3T3 using 27403-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27403-1-AP (RAB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue, Jurkat cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells
Positive IF detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27403-1-AP targets RAB5B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB,ELISA
Cited Applications FC, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RAB5B fusion protein Ag26584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB5B, member RAS oncogene family
Synonyms RAB5B, Ras related protein Rab 5B
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC032740
Gene Symbol RAB5B
Gene ID (NCBI) 5869
RRIDAB_2880862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB5B antibody 27403-1-APDownload protocol
IF protocol for RAB5B antibody 27403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

bioRxiv

The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
  • KD Validated
View Article
mouseWB

Proc Natl Acad Sci U S A

The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
View Article
human,mouseIHC,WB,FC

Mol Hum Reprod

Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis

Authors - Hairui Fan
View Article
mouseWB

Development

A crucial role for Arf6 in the response of commissural axons to Slit.

Authors - Mariko Kinoshita-Kawada
View Article