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RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10213-2-AP

Print datasheet

Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40

验证数据展示

  • WB analysis of HepG2 using 10213-2-AP

    WB analysis of HepG2 using 10213-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10213-2-AP

    IHC staining of human lung cancer using 10213-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 10213-2-AP

    IHC staining of human lung cancer using 10213-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10213-2-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10213-2-AP targets RABEPK/p40 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen RABEPK/p40 fusion protein Ag0285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rab9 effector protein with kelch motifs
Synonyms 40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000503
Gene Symbol RABEPK
Gene ID (NCBI) 10244
RRIDAB_2238057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
IHC protocol for RABEPK/p40 antibody 10213-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols