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RHEB Monoclonal antibody

RHEB Monoclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F9H8

Cat No : 66714-1-Ig

Print datasheet

Synonyms

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

验证数据展示

  • WB analysis of fetal human brain using 66714-1-Ig

    WB analysis of fetal human brain using 66714-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of human pancreas cancer using 66714-1-Ig

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse pancreas using 66714-1-Ig

    IF Staining of mouse pancreas using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human pancreas cancer using 66714-1-Ig

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, HeLa cells, DU 145 cells, mouse brain tissue
Positive IF detected inhuman pancreas cancer tissue, mouse pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66714-1-Ig targets RHEB in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity Human, mouse
Immunogen RHEB fusion protein Ag8887 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Ras homolog enriched in brain
Synonyms GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2
Calculated Molecular Weight 184 aa, 20 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC016155
Gene Symbol RHEB
Gene ID (NCBI) 6009
RRIDAB_2882065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

实验方案

Product Specific Protocols
WB protocol for RHEB antibody 66714-1-IgDownload protocol
IF protocol for RHEB antibody 66714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols