• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DPCD Polyclonal antibody

DPCD Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20936-1-AP

Print datasheet

Synonyms

DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4

验证数据展示

  • WB analysis using 20936-1-AP

    WB analysis using 20936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20936-1-AP (DPCD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20936-1-AP

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20936-1-AP

    IHC staining of mouse testis using 20936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20936-1-AP (DPCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20936-1-AP is DPCD Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DPCD fusion protein Ag15029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in a mouse model of primary ciliary dyskinesia
Synonyms DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC031695
Gene Symbol RP11-529I10.4
Gene ID (NCBI) 25911
RRIDAB_10734461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPCD (for deleted in a mouse model of primary ciliary dyskinesia), is predicted to code for a protein of 23 kDa which remains a novel candidate gene for PCD (Primary ciliary dyskinesia).

实验方案

Product Specific Protocols
WB protocol for DPCD antibody 20936-1-APDownload protocol
IHC protocol for DPCD antibody 20936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article