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Ribosomal protein L4 Monoclonal antibody

Ribosomal protein L4 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

FC, IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3C7A8

Cat No : 67028-1-Ig

Print datasheet

Synonyms

60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4

验证数据展示

  • WB analysis using 67028-1-Ig

    WB analysis using 67028-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 67028-1-Ig

    IHC staining of human lymphoma using 67028-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67028-1-Ig

    IF Staining of HepG2 using 67028-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 67028-1-Ig

    FC experiment of HepG2 using 67028-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67028-1-Ig targets Ribosomal protein L4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIP, WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein L4
Synonyms 60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009888
Gene Symbol RPL4
Gene ID (NCBI) 6124
RRIDAB_2882343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36578
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 67028-1-IgDownload protocol
IHC protocol for Ribosomal protein L4 antibody 67028-1-IgDownload protocol
IF protocol for Ribosomal protein L4 antibody 67028-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Authors - Matthias M Zimmer
View Article
mouseWB

Cell Rep

FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.

Authors - Sneha Shah
View Article