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- KD/KO Validated
RUVBL2 Polyclonal antibody
RUVBL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | C2C12 cells, Daudi cells, HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | human testis tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human heart tissue, human lymphoma tissue, human gliomas tissue, human spleen tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10195-1-AP targets RUVBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | RUVBL2 fusion protein Ag0253 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RuvB-like 2 (E. coli) |
Synonyms | 48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC000428 |
Gene Symbol | RUVBL2 |
Gene ID (NCBI) | 10856 |
RRID | AB_2184679 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y230 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IHC protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IF protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IP protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. | ||
Sci Adv Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.
| ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
J Clin Invest circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. | ||
Sci Adv ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer. | ||