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PLIN4 Polyclonal antibody

PLIN4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55404-1-AP

Print datasheet

Synonyms

Adipocyte protein S3 12, KIAA1881, Perilipin 4, PLIN4, S3 12, S3-12

验证数据展示

  • WB analysis of PC-3 using 55404-1-AP

    WB analysis of PC-3 using 55404-1-AP

    PC-3 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 55404-1-AP (S3-12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 55404-1-AP

    IHC staining of human pancreas using 55404-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55404-1-AP (S3-12 antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inPC-3 cells, Jurkat
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55404-1-AP targets PLIN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1881
Synonyms Adipocyte protein S3 12, KIAA1881, Perilipin 4, PLIN4, S3 12, S3-12
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 141 kDa
GenBank Accession NumberNM_001080400
Gene Symbol KIAA1881
Gene ID (NCBI) 729359
RRIDAB_2881321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96Q06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLIN4 (Perilipin 4) is a member of the perilipin family, a group of proteins that coat lipid droplets in adipocytes, the adipose tissue cells that are responsible for storing fat. The proteins in the Perilipin family are crucial regulators of lipid storage. PLIN4 expression is elevated in obese animals and humans. Plin4 is a lipid droplet protein (LDP) found predominantly in white adipose tissue (PMID: 23423172). It is lower expression in heart, skeletal muscle, and brown adipose tissue (PMID: 17878492).

实验方案

Product Specific Protocols
WB protocol for PLIN4 antibody 55404-1-APDownload protocol
IHC protocol for PLIN4 antibody 55404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Neurosci

TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.

Authors - Florent Laferrière
View Article
mouseWB

J Lipid Res

Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy.

Authors - Mika Yamada
View Article
humanIF

Ann Clin Transl Neurol

Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy

Authors - Qi Wang
View Article