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Alpha Antichymotrypsin Monoclonal antibody

Alpha Antichymotrypsin Monoclonal Antibody for FC, IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

FC, IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3F2F10

Cat No : 66078-1-Ig

Print datasheet

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

验证数据展示

  • WB analysis of human plasma using 66078-1-Ig

    WB analysis of human plasma using 66078-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount：0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human tonsillitis using 66078-1-Ig

    IHC staining of human tonsillitis using 66078-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA3/AACT antibody included
  • IHC staining of human tonsillitis using 66078-1-Ig

    IHC staining of human tonsillitis using 66078-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA3/AACT antibody included
  • IF Staining of human tonsillitis using 66078-1-Ig

    IF Staining of human tonsillitis using 66078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 66078-1-Ig

    FC experiment of HepG2 using 66078-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha Antichymotrypsin (66078-1-Ig, Clone:3F2F10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inhuman plasma, human colon tissue
Positive IHC detected inhuman tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINA3/AACT Ready-To-Use IHC Kit. SERPINA3/AACT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66078-1-Ig is Alpha Antichymotrypsin Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, pig
Cited Reactivityhuman
Immunogen Alpha Antichymotrypsin fusion protein Ag2830 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Synonyms A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3
Calculated Molecular Weight 448 aa, 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC010530
Gene Symbol SERPINA3
Gene ID (NCBI) 12
RRIDAB_11182502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

实验方案

Product Specific Protocols
WB protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
IHC protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
IF protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
FC protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
View Article
humanWB,IHC

Breast Cancer

Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.

Authors - Yingzi Zhang
View Article
humanIHC

Pharmaceutics

Distribution of Monocarboxylate Transporters in Brain and Choroid Plexus Epithelium

Authors - Masaki Ueno
View Article