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- KD/KO Validated
SETDB1 Polyclonal antibody
SETDB1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, PC-3 cells, human testis tissue, MCF-7 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11231-1-AP targets SETDB1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | ChIP, CoIP, IF, IHC, IP, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | SETDB1 fusion protein Ag1725 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SET domain, bifurcated 1 |
| Synonyms | ESET, H3 K9 HMTase 4, H3 K9 HMTase4, KG1T, KIAA0067, KMT1E, Lysine N methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1, SETDB1 |
| Calculated Molecular Weight | 143 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC009362 |
| Gene Symbol | SETDB1 |
| Gene ID (NCBI) | 9869 |
| RRID | AB_2186069 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15047 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SETDB1 antibody 11231-1-AP | Download protocol |
| IHC protocol for SETDB1 antibody 11231-1-AP | Download protocol |
| IF protocol for SETDB1 antibody 11231-1-AP | Download protocol |
| IP protocol for SETDB1 antibody 11231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.
| ||
Nature Genome surveillance by HUSH-mediated silencing of intronless mobile elements.
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Science GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
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