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CHT1 Polyclonal antibody

CHT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21848-1-AP

Print datasheet

Synonyms

CHT, CHT1, hCHT, SLC5A7

验证数据展示

  • WB analysis of mouse brain using 21848-1-AP

    WB analysis of mouse brain using 21848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21848-1-AP (CHT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 21848-1-AP

    IHC staining of human brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

  • IHC staining of human brain using 21848-1-AP

    IHC staining of human brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 21848-1-AP (CHT1 antibody at dilution of 1:50.

  • IHC staining of rat brain using 21848-1-AP

    IHC staining of rat brain using 21848-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21848-1-AP (CHT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 21848-1-AP

    IP experiment of mouse heart using 21848-1-AP

    IP result of anti-CHT1 (IP:21848-1-AP, 4ug; Detection:21848-1-AP 1:500) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21848-1-AP is CHT1 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CHT1 fusion protein Ag16136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (choline transporter), member 7
Synonyms CHT, CHT1, hCHT, SLC5A7
Calculated Molecular Weight 580 aa, 63 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC111525
Gene Symbol SLC5A7
Gene ID (NCBI) 60482
RRIDAB_2878925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHT1 antibody 21848-1-APDownload protocol
IHC protocol for CHT1 antibody 21848-1-APDownload protocol
IP protocol for CHT1 antibody 21848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Mol Metab

Activation of a non-neuronal cholinergic system in visceral white adipose tissue of obese mice and humans

Authors - Ilenia Severi
View Article