STAT2 Monoclonal antibody
STAT2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G12C4
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, A549 cells, PC-3 cells, COLO 320 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy STAT2 Ready-To-Use IHC Kit. STAT2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66485-1-Ig targets STAT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF, IP, WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Immunogen | STAT2 fusion protein Ag10168 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | signal transducer and activator of transcription 2, 113kDa |
| Synonyms | ISGF 3, P113, STAT113, STAT2 |
| Calculated Molecular Weight | 851 aa, 98 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC051284 |
| Gene Symbol | STAT2 |
| Gene ID (NCBI) | 6773 |
| RRID | AB_2881851 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P52630 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STAT2 antibody 66485-1-Ig | Download protocol |
| IHC protocol for STAT2 antibody 66485-1-Ig | Download protocol |
| IF protocol for STAT2 antibody 66485-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Silencing SIRT1 promotes the anti-HBV action of IFN-α by regulating Pol expression and activating the JAK-STAT signaling pathway | ||
Exp Eye Res Integrated analysis of murine cornea identifies JAK/STAT signaling pathway upregulated specifically in female Vitamin A Deficient mice | ||
PLoS Pathog Free ISG15 inhibits Pseudorabies virus infection by positively regulating type I IFN signaling | ||
J Nanobiotechnology Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy |