- Featured Product
- KD/KO Validated
Syntaxin 7 Polyclonal antibody
Syntaxin 7 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, canine and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, MDCK cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12322-1-AP targets Syntaxin 7 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | CoIP, IF, IHC, WB |
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Syntaxin 7 fusion protein Ag2975 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | syntaxin 7 |
| Synonyms | STX7, syntaxin 7 |
| Calculated Molecular Weight | 261 aa, 30 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC011975 |
| Gene Symbol | STX7 |
| Gene ID (NCBI) | 8417 |
| RRID | AB_2239979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IHC protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IF protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IP protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. | ||
J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes
| ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. | ||
Cell Logist Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins. | ||
J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome |
