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SYK Monoclonal antibody

SYK Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

FC, IF, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C4A12

Cat No : 66721-1-Ig

Print datasheet

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK



经过测试的应用

Positive WB detected inRaji cells, THP-1 cells, Ramos cells, Daudi cells
Positive IP detected inMDA-MB-231 cells
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66721-1-Ig targets SYK in WB, IP, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen SYK fusion protein Ag17654 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name spleen tyrosine kinase
Synonyms DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK
Calculated Molecular Weight 635 aa, 72 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_2882072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

实验方案

Product Specific Protocols
WB protocol for SYK antibody 66721-1-IgDownload protocol
IF protocol for SYK antibody 66721-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
humanWB

Exp Cell Res

CD93 promotes acute myeloid leukemia development and is a potential therapeutic target

Authors - Jie Jia