- Featured Product
- KD/KO Validated
SYK Monoclonal antibody
SYK Monoclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (2)
Applications
FC, IF, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4C4A12
验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, THP-1 cells, Ramos cells, Daudi cells |
Positive IP detected in | MDA-MB-231 cells |
Positive IF detected in | human tonsillitis tissue |
Positive FC detected in | K-562 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
66721-1-Ig targets SYK in WB, IP, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF, IP, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | SYK fusion protein Ag17654 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | spleen tyrosine kinase |
Synonyms | DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK |
Calculated Molecular Weight | 635 aa, 72 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC001645 |
Gene Symbol | SYK |
Gene ID (NCBI) | 6850 |
ENSEMBL Gene ID | ENSG00000165025 |
RRID | AB_2882072 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P43405 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SYK antibody 66721-1-Ig | Download protocol |
IF protocol for SYK antibody 66721-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Exp Cell Res CD93 promotes acute myeloid leukemia development and is a potential therapeutic target |