TGF Beta 1 Polyclonal antibody
TGF Beta 1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18978-1-AP targets TGF Beta 1 in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | Cell treatment, IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sheep, pig, canine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transforming growth factor, beta 1 |
| Synonyms | CED, DPD1, LAP, TGF beta 1, TGFB, TGFB1, TGFbeta, TGF-beta 1, TGF-beta1, TGFβ, TGFβ1, TGF-β1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | NM_000660 |
| Gene Symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGF Beta 1 antibody 18978-1-AP | Download protocol |
| IHC protocol for TGF Beta 1 antibody 18978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Circulation Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation. | ||
Biomaterials A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration | ||
Br J Dermatol Intense Foxp3(+) CD25(+) regulatory T cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8(+) /Foxp3(+) CD25(+) ratio. | ||
Proc Natl Acad Sci U S A miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression. | ||
