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  • KD/KO Validated

TMEM173/STING Monoclonal antibody

TMEM173/STING Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat and More (1)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1F1E1

Cat No : 66680-1-Ig

Print datasheet

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, HEK-293 cells, THP-1 cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66680-1-Ig targets TMEM173/STING in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity Human, Rat
Cited Reactivityhuman
Immunogen TMEM173/STING fusion protein Ag20363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC047779
Gene Symbol TMEM173
Gene ID (NCBI) 340061
RRIDAB_2882034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

实验方案

Product Specific Protocols
WB protocol for TMEM173/STING antibody 66680-1-IgDownload protocol
IHC protocol for TMEM173/STING antibody 66680-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Immunity

Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING.

Authors - Run Fang
mouseWB

Cell Death Differ

The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.

Authors - Miao Zhao
  • KO Validated
mouseWB

Cell Death Differ

Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.

Authors - Ming Zou
human,mouseIF

Autophagy

Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.

Authors - Kunpeng Liu
  • KO Validated
humanIF

Oncogene

GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair.

Authors - Hui Xu
mouseIF

Free Radic Biol Med

STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury

Authors - Lini Jin