TNFAIP3 Monoclonal antibody

TNFAIP3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (2)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A11G6

Cat No : 66695-1-Ig

Print datasheet

Synonyms

A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20



经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue, human thyroid cancer tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66695-1-Ig targets TNFAIP3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman, mouse
Immunogen TNFAIP3 fusion protein Ag18150 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 3
Synonyms A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20
Calculated Molecular Weight 790 aa, 90 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2882048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

实验方案

Product Specific Protocols
WB protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
IHC protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
IF protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
FC protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biology (Basel)

Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo

Authors - Stamatia Papoutsopoulou
humanWB,IHC,IF

Cell Death Dis

A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.

Authors - Yilu Feng
human,mouseIHC,IF

Sci Total Environ

Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes

Authors - Yinzhao Jia