• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TNFRSF17 Polyclonal antibody

TNFRSF17 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 27724-1-AP

Print datasheet

Synonyms

B cell maturation protein, BCM, BCMA, CD269, TNFRSF17

验证数据展示

  • IHC staining of human breast cancer using 27724-1-AP

    IHC staining of human breast cancer using 27724-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Daudi using 27724-1-AP

    FC experiment of Daudi using 27724-1-AP

    1X10^6 Daudi cells were stained with 0.20 ug/test Anti-Human TNFRSF17 (27724-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

  • WB analysis of Daudi using 27724-1-AP

    WB analysis of Daudi using 27724-1-AP

    Daudi and Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inDaudi cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27724-1-AP targets TNFRSF17 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen TNFRSF17 fusion protein Ag27049 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 17
Synonyms B cell maturation protein, BCM, BCMA, CD269, TNFRSF17
Calculated Molecular Weight 184 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC058291
Gene Symbol TNFRSF17
Gene ID (NCBI) 608
RRIDAB_2880954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02223
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFRSF17 (also known as BCMA and CD269) is located on chromosome 16p13.1 and was initially cloned as a translocation product in human T cell lymphom. TNFRSF17 is preferentially expressed in mature B lymphocytes. TNFRSF17 is involved in the late stages of B cell maturation; it is important for the survival of plasmablasts and long-lived plasma cells in the bone marrow.

实验方案

Product Specific Protocols
WB protocol for TNFRSF17 antibody 27724-1-APDownload protocol
IHC protocol for TNFRSF17 antibody 27724-1-APDownload protocol
FC protocol for TNFRSF17 antibody 27724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Med

Developing high-affinity decoy receptors to treat multiple myeloma and diffuse large B cell lymphoma.

Authors - Yu Rebecca Miao
View Article