• Featured Product
  • KD/KO Validated

TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

FC, IF, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1D1E1

Cat No : 66498-1-Ig

Print datasheet

Synonyms

RING finger protein 85, RNF85, TRAF6



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HepG2 cells, U2OS cells
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66498-1-Ig targets TRAF6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity Human
Cited Reactivityhuman, fish, pig
Immunogen TRAF6 fusion protein Ag24696 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 6
Synonyms RING finger protein 85, RNF85, TRAF6
Calculated Molecular Weight 522 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
RRIDAB_2881862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4K3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

实验方案

Product Specific Protocols
WB protocol for TRAF6 antibody 66498-1-IgDownload protocol
IHC protocol for TRAF6 antibody 66498-1-IgDownload protocol
IF protocol for TRAF6 antibody 66498-1-IgDownload protocol
FC protocol for TRAF6 antibody 66498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Autophagy

Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis

Authors - Jiajun Liu
humanWB

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
humanWB

Cell Death Differ

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Authors - Shunfeng Hu
humanIHC

J Immunother Cancer

Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.

Authors - Yuwei Deng
humanWB

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
WB

Theranostics

Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.

Authors - Junjian Hu