TRIM26 Polyclonal antibody

TRIM26 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27013-1-AP

Print datasheet

Synonyms

Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173



经过测试的应用

Positive WB detected inHEK-293T cells, mouse testis tissue, RAW 264.7 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRIM26 Ready-To-Use IHC Kit. TRIM26 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27013-1-AP is TRIM26 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Immunogen TRIM26 fusion protein Ag25546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 26
Synonyms Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC024039
Gene Symbol TRIM26
Gene ID (NCBI) 7726
RRIDAB_2880721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12899
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

实验方案

Product Specific Protocols
WB protocol for TRIM26 antibody 27013-1-APDownload protocol
IHC protocol for TRIM26 antibody 27013-1-APDownload protocol
IP protocol for TRIM26 antibody 27013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma

Authors - Zhangjie Wang
pigWB

Virus Res

TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection.

Authors - Pandeng Zhao
mouseWB

Cell Death Dis

USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression

Authors - Weiwei Wang
humanWB,IHC,IF,IP

Int J Biol Sci

TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation

Authors - Yuening Sun
  • KD Validated
humanWB,IF

FASEB J

TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β

Authors - Mingjiao Zhang
  • KD Validated