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TRIP6 Polyclonal antibody

TRIP6 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21163-1-AP

Print datasheet

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

验证数据展示

  • WB analysis of HeLa using 21163-1-AP

    WB analysis of HeLa using 21163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21163-1-AP (TRIP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21163-1-AP

    IF Staining of HepG2 using 21163-1-AP

    Immunofluorescent analysis of HepG2 cells, using TRIP6 antibody 21163-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human testis tissue, mouse lung tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21163-1-AP is TRIP6 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen TRIP6 fusion protein Ag15149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyroid hormone receptor interactor 6
Synonyms OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1
Calculated Molecular Weight 476 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC004249
Gene Symbol TRIP6
Gene ID (NCBI) 7205
RRIDAB_10732813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15654
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

实验方案

Product Specific Protocols
WB protocol for TRIP6 antibody 21163-1-APDownload protocol
IF protocol for TRIP6 antibody 21163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols