beta Tubulin Antibody 42 Publications

Rabbit Polyclonal| Catalog number: 10094-1-AP

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Species specificity:
human, mouse, rat, hamsters

Positive WB detected in:
HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, mouse brain tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
rat brain tissue, human cerebellum tissue, human colon tissue, rat brain tissue, rat testis tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tubulin, beta 3

Calculated molecular weight:
50 kDa

Observed molecular weight:
55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TUBB3

Synonyms
beta tubulin, TUBB
Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.


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