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  • KD/KO Validated

ZHX2 Polyclonal antibody

ZHX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20136-1-AP

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Synonyms

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse ovary tissue, mouse lung tissue, rat brain tissue, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20136-1-AP targets ZHX2 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsChIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ZHX2 fusion protein Ag14088 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc fingers and homeoboxes 2
Synonyms AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight 92-100 kDa
GenBank Accession NumberBC042145
Gene Symbol ZHX2
Gene ID (NCBI) 22882
RRIDAB_10666438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6X8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

实验方案

Product Specific Protocols
WB protocol for ZHX2 antibody 20136-1-APDownload protocol
IHC protocol for ZHX2 antibody 20136-1-APDownload protocol
IF protocol for ZHX2 antibody 20136-1-APDownload protocol
IP protocol for ZHX2 antibody 20136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Cancer

HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.

Authors - Xiaojia Song
humanWB

J Pathol

ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p.

Authors - Xiangguo Yu
mouseWB

J Immunol

Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.

Authors - Zehua Wang
  • KO Validated
humanWB,IHC

Cancer Cell Int

ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.

Authors - Yankun Zhang
  • KD Validated
humanWB

Biochem Biophys Res Commun

Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.

Authors - Fang Wan
  • KD Validated
humanWB,IHC

FEBS Open Bio

Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.

Authors - Anqi Cheng