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  • KD/KO Validated

Beta Arrestin 2 Polyclonal antibody

Beta Arrestin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10171-1-AP

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Synonyms

ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2, β-Arrestin 2



经过测试的应用

Positive WB detected inmouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue
Positive IHC detected inhuman tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse Retina tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Xenopus
Immunogen Beta Arrestin 2 fusion protein Ag0227 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arrestin, beta 2
Synonyms ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2, β-Arrestin 2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007427
Gene Symbol ARRB2
Gene ID (NCBI) 409
RRIDAB_10644158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

实验方案

Product Specific Protocols
WB protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IHC protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IF protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.

Authors - Yuqing Wu
  • KO Validated
ratWB

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew
human,mouseWB,IF,CoIP

J Pharm Anal

Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis

Authors - Jia-Jia Zhang
  • KD Validated
mouseIF

J Cell Biol

BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.

Authors - Fan Ye
mouseIF

Acta Pharmacol Sin

Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy.

Authors - Tian-Tian Wei
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger