APP Polyclonal antibody
APP Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse , rat
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | fetal human brain tissue, U-251 cells, HEK-293T cells, mouse brain tissue, rat brain tissue, HeLa cells |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
27320-1-AP targets APP in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse , rat |
Cited Reactivity | human |
Immunogen | APP fusion protein Ag26299 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | amyloid beta (A4) precursor protein |
Synonyms | A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta |
Calculated Molecular Weight | 87 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC004369 |
Gene Symbol | APP |
Gene ID (NCBI) | 351 |
RRID | AB_2880842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05067 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for APP antibody 27320-1-AP | Download protocol |
IHC protocol for APP antibody 27320-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Alzheimers Res Ther Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing. |