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Dynamitin (1-86 AA) Polyclonal antibody

Dynamitin (1-86 AA) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat No : 10032-1-AP

Print datasheet

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

验证数据展示

  • WB analysis of mouse lung using 10032-1-AP

    WB analysis of mouse lung using 10032-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue, human heart tissue, human testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10032-1-AP targets Dynamitin (1-86 AA) in WB,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,ELISA
Tested Reactivity human, mouse
Immunogen Dynamitin (1-86 AA) fusion protein Ag0029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynactin 2 (p50)
Synonyms DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberAY189155
Gene Symbol DCTN2
Gene ID (NCBI) 10540
RRIDAB_2090611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13561
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 86aa of full length p50 dynamitin.

实验方案

Product Specific Protocols
WB protocol for Dynamitin (1-86 AA) antibody 10032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols