GFP tag Monoclonal antibody
GFP tag Monoclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein and More (5)
Applications
FC, IF, IP, WB, ELISA and More (4)
Conjugate
Unconjugated
416
CloneNo.
1E10H7
验证数据展示
经过测试的应用
Positive WB detected in | GFP transgenic mouse brain tissue, Recombinant protein |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF detected in | Transfected HEK-293 cells |
Positive FC detected in | Transfected HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66002-1-Ig targets GFP tag in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | FC, IF, IP, WB, ELISA |
Cited Applications | ChIP, CoIP, ELISA, FC, IF, IHC, IP, RIP, WB |
Tested Reactivity | recombinant protein |
Cited Reactivity | human, mouse, rat, zebrafish, pig |
Immunogen | GFP tag fusion protein Ag2128 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | GFP tag |
Synonyms | CFP, eGFP, eYFP, GFP, GFP tag, YFP |
Calculated Molecular Weight | 26 kDa |
GenBank Accession Number | U73901 |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_11182611 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Green fluorescence protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) derived from the jellyfish Aequorea Victoria which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP, when exposed to light in the blue to ultraviolet spectrum, will show a bright green fluorescent light, making it a very useful tool in research.
What is the molecular weight of GFP?
26.9 kDa
How does GFP work?
GFP was first isolated from the jellyfish Aequorea Victoria, a source of bioluminescence, in the 1960s and in 2008 the Nobel Prize in Chemistry was awarded "for the discovery and development of the green fluorescent protein, GFP" to Osamu Shimomura and colleagues, who recognized its potential in research (PMID: 13911999). A short amino acid sequence within the protein acts as the chromophore, which absorbs UV light at 395 nm and emits green light at 509 nm.
Why is GFP a useful reporter?
When GFP was sequenced in 1992 (PMID: 1347277) it allowed scientists to express it in other organisms using transgenic techniques. It does not require cofactors to work, is non-toxic to live cells, and is relatively small, making it ideal as a "tag" for other proteins, identifiable by shining a UV light and observing the green fluorescence. The tertiary folded structure of GFP forms a chromophore at the center of a barrel shape, which protects the fluorescence-emitting amino acid chain from solvents, meaning it can function in many environments (PMID 9759496).
What are the applications for GFP?
When expressed attached to another protein, GFP can be used as a reporter gene to measure expression levels or can easily be used in fluorescence microscopy. It has been used to highlight proteins in a variety of model organisms, including bacteria, zebrafish, and mice.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GFP tag antibody 66002-1-Ig | Download protocol |
IF protocol for GFP tag antibody 66002-1-Ig | Download protocol |
IP protocol for GFP tag antibody 66002-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. | ||
Cell Stem Cell DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. | ||
Nat Neurosci Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1. | ||
Nat Biomed Eng Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. |