alpha Tubulin Antibody 49 Publications

Mouse Monoclonal| Catalog number: 66031-1-Ig |CloneNo.: 1E4C11

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Species specificity:
human, mouse, rat, dog, rice

Positive WB detected in:
multi-cells, HeLa

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human tonsillitis tissue, human liver cancer tissue, human testis tissue, human tonsillitis tissue

Positive IF detected in:
HepG2 cells, MCF-7 cells, Hela cells, MDCK cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tubulin, alpha 1b

Calculated molecular weight:
50 kDa

Observed molecular weight:
52 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TUBA1B

Synonyms
alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, tubulin, alpha 1b
Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antibody recognizes Tubulin Alpha only.


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