Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OCT4/POU5F1 antibody (11263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human OCT4/POU5F1 (11263-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
