Various lysates were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB result of PCGF6 antibody (24103-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCGF6 transfected HEK-293 cells.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human PCGF6 (24103-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
PC-13 cells were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.