Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24418-1-AP (CHAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

rat brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP Result of anti-CHAT (IP:24418-1-AP, 4ug; Detection:24418-1-AP 1:300) with rat brain tissue lysate 4000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IP Result of anti-SH2D3C (IP:12882-1-AP, 3ug; Detection:12882-1-AP 1:500) with mouse brain tissue lysate 6000ug.

IP result of SH2D3C antibody (12882-1-AP for IP and Detection) with mouse brain tissue lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 12882-1-AP (SH2D3C antibody) at dilution of 1:100 (under 10x lens).

CHAT fusion protein Ag19602 SDS-PAGE