Various lysates were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse heart tissue lysate 3600 ug.