rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug.
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).