Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001 (GST Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were stained with 0.20 ug PSMD9 antibody (26922-1-AP, red) and 0.20 ug Rabbit IgG control (10000-0-AP, blue). Fixed with 90% MeOH.

Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions.

GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours.

IP result of anti-GST Tag (IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug.

Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens).

IP Result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Western blot analysis of cell extract from BL21 cells expressing GST

Western blot, dilution series of purified recombinant GST-protein, anti-GST antibody

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-GST Tag (CL647-10000) (blue), or 0.4 ug CoraLite®488-conjugated TRAP1 Rabbit PolyAb (CL488-10325, Clone: ) (red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GST tagged protein and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.