Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IP Result of anti-NCL (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug.
1X10^6 HeLa cells were stained with 0.20ug NCL antibody (10556-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.