mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-NMDAR2B/GRIN2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of SH-SY5Y cells using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2B/GRIN2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66565-1-PBS in a different storage buffer formulation.