Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP result of anti-SIX2 (IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.
IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".
IF result of SIX2 antibody (11562-1-AP, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) incondense metanephric mesenchyme surrounding the ureteric bud tip.