CoraLite® Plus 647-conjugated AIF Monoclonal antibody
AIF Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
8C12B2
验证数据展示
经过测试的应用
| Positive IF detected in | human kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67791-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-67791 targets AIF in IF applications and shows reactivity with Human samples.
| Tested Applications | IF-P |
| Tested Reactivity | Human |
| Immunogen | AIF fusion protein Ag12400 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 1 |
| Synonyms | AIF, AIFM1, Mitochondrion Marker, PDCD8 |
| Calculated Molecular Weight | 609 aa, 66 kDa |
| Observed Molecular Weight | 67 kDa, 57 kDa |
| GenBank Accession Number | BC111065 |
| Gene Symbol | AIFM1 |
| Gene ID (NCBI) | 9131 |
| RRID | AB_2920311 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95831 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 AIF antibody CL647-67791 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |